文献类型：期刊文献

英文题名：Comparative Transcriptome Analysis Reveals Stem Secondary Growth of Grafted Rosa rugosa 'Rosea' Scion and R. multiflora 'Innermis' Rootstock

作者：Sun, Jing-shuang[1] Hu, Rui-yang[1] Lv, Fu-ling[1] Yang, Yan-fang[2] Tang, Zhi-min[1] Zheng, Guang-shun[1] Li, Jian-bo[1] Tian, Hua[1] Xu, Yan[1] Li, Shao-feng[1]

通信作者：Li, SF[1]|[a00055e7457cb6f03b07f]李少锋;

机构：[1]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 102300, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Beijing 102300, Peoples R China

年份：2020

卷号：11

期号：2

外文期刊名：GENES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000519271500040)】；

基金：This research was funded by the Fundamental Research Funds for the Central Non-profit Research Institution of CAF, grant number CAFYBB2016MA008.

语种：英文

外文关键词：RNA-sequencing; Rosa; grafting; stem; scion; rootstock

摘要：Grafted plant is a chimeric organism formed by the connection of scion and rootstock through stems, so stem growth and development become one of the important factors to affect grafted plant state. However, information regarding the molecular responses of stems secondary growth after grafting is limited. A grafted Rosa plant, with R. rugosa 'Rosea' as the scion (Rr_scion) grafted onto R. multiflora 'Innermis' as the stock (Rm_stock), has been shown to significantly improve stem thickness. To elucidate the molecular mechanisms of stem secondary growth in grafted plant, a genome-wide transcription analysis was performed using an RNA sequence (RNA-seq) method between the scion and rootstock. Comparing ungrafted R. rugosa 'Rosea' (Rr) and R. multiflora 'Innermis' (Rm) plants, there were much more differentially expressed genes (DEGs) identified in Rr_scion (6887) than Rm_stock (229). Functional annotations revealed that DEGs in Rr_scion are involved in two Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways: the phenylpropanoid biosynthesis metabolism and plant hormone signal transduction, whereas DEGs in Rm_stock were associated with starch and sucrose metabolism pathway. Moreover, different kinds of signal transduction-related DEGs, e.g., receptor-like serine/threonine protein kinases (RLKs), transcription factor (TF), and transporters, were identified and could affect the stem secondary growth of both the scion and rootstock. This work provided new information regarding the underlying molecular mechanism between scion and rootstock after grafting.