文献类型：期刊文献

中文题名：高效毛细管电泳法检测印楝素A的初步研究

英文题名：Preliminary Study on Determination of Azadirachtin A by High-performance Capillary Electrophoresis

作者：王有琼[1] 马李一[1] 张重权[1] 段琼芬[1]

第一作者：王有琼

机构：[1]中国林业科学研究院资源昆虫研究所

年份：2007

卷号：27

期号：B10

起止页码：85-88

中文期刊名：林产化学与工业

外文期刊名：Chemistry and Industry of Forest Products

收录：CSTPCD;;Scopus;CSCD:【CSCD_E2011_2012】；

基金：国家科技支撑计划重点项目(2006BAD18B04);国家林业局"948"引进项目(2002-58)

语种：中文

中文关键词：高效毛细管电泳;印楝素A;检测;缓冲溶液

外文关键词：high performance capillary electrophoresis ; azadirachtin A; determination; buffer solution

分类号：TQ91;Q503

摘要：从理论角度分析了高效毛细管电泳技术检测印楝素A的可能性,并以试验进行证明。在十二烷基硫酸钠(SDS)浓度为25 mmol/L、0.02 mol/L pH值6.86混合磷酸盐缓冲溶液,甲醇-缓冲溶液(体积比7∶3)作为运行缓冲液,分离电压20 kV下得到稳定的电流曲线,二极管阵列检测器(PDA)检测波长214 nm,在迁移时间2.6 min处检测到印楝素A的电泳特征峰,电泳谱图面积积分结果和高效液相色谱分析结果无显著差异。
The possibility of using high-performance capillary electrophoresis （HPCE） to determine azadirachtin A was analysed theoretically, and proved experimentally. The stable current curve had been obtained under the testing condition as follows： SDS concentration 25 mmol/L, phosphate buffer solution concentration 0.02 mol/L, pH value 6.86, volume ratio of methanol-buffer solution 7 ： 3, separation voltage 20 kV. The characteristic electrophoretogram of azadirachtin A was very well examinated by PDA at wavelengh 214 nm and migration time 2.6 rain. The result of area integration has no notable difference with that of HPLC.