文献类型：期刊文献

中文题名：超声波提取牡丹籽壳多酚工艺响应面法优化及抗氧化性研究

英文题名：Optimization of ultrasound-assisted extraction of polyphenols from peony seed shell by response surface methodology and its antioxidant activity

作者：张红玉[1,2] 王成章[1,2,3] 张宇思[1,2] 陶冉[1,2] 原姣姣[1,2] 李文君[1,2] 朱佳妮[4]

第一作者：张红玉

机构：[1]中国林业科学研究院林产化学工业研究所,南京210042;[2]生物质化学利用国家工程实验室,国家林业局林产化学工程重点开放性实验室,江苏省生物质能源与材料重点实验室,南京210042;[3]中国林业科学研究院林业新技术研究所,北京100091;[4]南京林业大学化工学院,南京210037

年份：2015

卷号：40

期号：6

起止页码：90-94

中文期刊名：中国油脂

外文期刊名：China Oils and Fats

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

语种：中文

中文关键词：牡丹籽壳;多酚;超声波提取;响应面法;抗氧化性

外文关键词：peony seed shell; polyphenols ;ultrasound- assisted extraction; response surfacemethodology ; antioxidant activity

分类号：TS229

摘要：采用超声波提取牡丹籽壳多酚,以多酚含量为指标,通过单因素实验分别考察乙醇体积分数、液料比、提取时间和提取温度的影响,并采用响应面法获得最优的超声波提取牡丹籽壳多酚的工艺条件。以抗氧化剂(VC和BHT)为参照,采用DPPH和FRAP法评估牡丹籽壳多酚的抗氧化性。结果表明,超声波提取牡丹籽壳多酚最优工艺条件为:超声波功率100 W,乙醇体积分数70%,液料比25∶1,提取时间80 min,提取温度60℃;在最优条件下,多酚含量为5.75%。牡丹籽壳多酚清除DPPH自由基的IC50为71.0μg/m L,FRAP值为18.33 mmol/L,具有一定的抗氧化作用。
Polyphenols was extracted from peony seed shell by ultrasound- assisted method, and the effects of volume fraction of ethanol, ratio of liquid to solid, extraction time and extraction temperature were studied by single factor experiment with content of polyphenols as index, then the extraction condi- tions of polyphenols from peony seed shell were optimized by response surface methodology. Using Vc and BHT as control, the antioxidant activity of polyphenols from peony seed shell was assessed by DPPH and FRAP methods. The results demonstrated that the optimal ultrasound - assisted extraction conditions of polyphenols from peony seed shell were obtained as follows： ultrasonic power 100 W, volume fraction of ethanol 70% , ratio of liquid to solid 25 ： 1, extraction time 80 rain, extraction temperature 60 ℃ ; under these conditions, the content of polyphenols was 5.75%. The ICso value of polyphenols from peony seed shell on scavenging DPPH free radical was 71.0 p,g/mL and its FRAP value was 18.33 retool/L, which indicated that the Dolvohenols from peony seed shell had certain antioxidant activity.