文献类型：期刊文献

中文题名：白蜡虫蜡酯合酶在昆虫细胞Sf9中的表达

英文题名：Expression of Ericerus pela Wax Ester Synthase in Spodoptera frugiperda Sf9 Cell Line

作者：亓倩[1] 于淑惠[1] 孙涛[1] 王雪庆[1] 刘博文[1] 杨璞[1] 陈晓鸣[1]

第一作者：亓倩

机构：[1]中国林业科学研究院资源昆虫研究所,国家林业局资源昆虫培育与利用重点实验室,云南昆明650224

年份：2016

卷号：29

期号：2

起止页码：191-195

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：林业公益性行业科研专项201504302,201304808;云南省应用基础研究重点项目2013FA052;国家自然科学基金31572337;国家863计划2014AA021801

语种：中文

中文关键词：白蜡虫;蜡酯合酶;载体构建;Bac-to-Bac表达系统;Sf9细胞系

外文关键词：Ericerus pela; wax ester synthase; Construction of expression vector; bac-to-bac expression vector sys tem; Sf9 cells lines.

分类号：S899.1

摘要：[目的]本研究欲利用Bac-to-Bac表达系统,在Sf9细胞中表达WS。[方法]将WS编码序列克隆到质粒p FastBac^(TM)HT B后,转化大肠杆菌DH10Bac^(TM),经抗性和蓝白斑筛选,获得重组杆粒r Bacmid/Epel WS。将r Bacmid/EpelWS转染Sf9细胞。[结果]免疫荧光标记检测表明:WS在受感染细胞的细胞质中表达。[结论]本研究成功表达了白蜡虫WS,为白蜡虫WS的生化特性研究奠定了基础。
[Objective]Use Bac-to-Bac expression system to express WS in Sf9 cell line. [Method]The WS coding sequence was cloned into plasmid p Fast Bac^（TM）HT B,and then transformed Escherichia coli DH10 Bac^（TM）. After resistant and blue-white screening,the recombinant bacmid r Bacmid / Epel WS were obtained. The r Bacmid / Epel WS was transfected into Sf9 cells. [Result]The Immunofluorescence analysis showed that the target protein was expressed in the cytoplasm of the infected cells. [Conclusion]The WS was expressed successfully,it lays the foundation for further research on the biochemical characteristics of WS of the Ericerus pela.