文献类型：期刊文献

中文题名：茶油多酚的碳纳米管富集提取方法研究

英文题名：Analysis on Polyphenol Extraction in Oil-Tea Camellia Seed Oil by Accumulation of CNTs

作者：许伟[1,2] 罗凡[1] 仲山民[2,3] 费学谦[1] 戚雨婷[1,2]

第一作者：许伟

机构：[1]中国林业科学研究院亚热带林业研究所;[2]浙江农林大学农业与食品科学学院;[3]漳州城市职业学院食品工程系

年份：2017

卷号：32

期号：11

起止页码：76-82

中文期刊名：中国粮油学报

外文期刊名：Journal of the Chinese Cereals and Oils Association

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：中央级公益性科研院所基本科研业务费专项资金(CAFYBB2014QA007)

语种：中文

中文关键词：茶油;多酚;碳纳米管

外文关键词：oil -tea camellia seed oil, polyphenol, carbon nanotubes

分类号：TS229

摘要：本研究建立了一种利用碳纳米管(Carbon Nanotubes,CNTs)富集茶油中多酚新型前处理方法。在考察CNTs物化性质、吸附介质、解吸溶液等对多酚吸附、解吸规律的基础上,采用经纯化的管径在20~30nm、携带-COOH的CNTs为吸附剂,1∶10的料液比,以100 r/min、10℃恒温振荡2 h为吸附条件,以丙酮为解吸液进行超声辅助解吸,结果发现:采用CNTs富集茶油中的多酚,用于补偿不完全吸附和解吸的补偿因子为2.032。当茶油中的多酚质量浓度在4.4~93.0μg/g,富集后的解吸液的吸光度与茶油多酚浓度呈线性关系,相关系数(r2)为0.935 1,方法最低检出限为3.24μg/g,加标回收率为87.99%~110.95%,相对标准偏差(RSD,n=6)为8.38%~10.74%,校正后的检测结果与固相萃取法的结果无显著性差异(P>0.05)。研究结果表明采用CNTs为吸附剂富集、分析茶油中的多酚成分具有稳定性好、准确度高等优点,为茶油多酚的检测提供了一条新途径。
This study developed a new pre - treatment method of polyphenol in oil - tea camellia seed oil through the accumulation of CNTs. On the basis of investigating the physico - chemical property of CNTs, adsorption medium, desorption solution on the adsorption and desorption of polyphenol standard product, we took purified CNTs as adsorbent, which were 20 - 30 nm in diameter and carried much - COOH, 1 ： 10 as materiaL/liquid ratio, and the adsorption conditions were as follows： shaking table at constant temperature of 10 ℃ by 2 h, the velocity was 100r/ min. After that CNTs were desorpted by 20 mL actone added with ultrasonic, which repeated 3 times. Finally we found that the compensate factor of the method which was used to compensate for incomplete adsorption and desorp- tion was 2. 032. When the concentration of polyphenol in oil - tea camellia seed oil was between 4.4 and 93.0 p,g,/g, the absorbanee of desorption solution presented a good linear relationship with the concentration of polyphenol in oil - tea camellia seed oil, the correlation coefficient （r2） was 0.935 1. The minimum determination limit of the method was 3.24 μg/g and the recoveries were between 87.99% and 110.95% with relative standard deviation （ RSD, n = 6） between 8.38% and 10.74%. There＇s no significant difference between the results of this method and those of Dio - SPE （P 〉 0.05 ）. The results showed that the method which taken CNTs as adsorbent to analysis the polyphenol in oil - tea camellia seed oil had the advantage of high stability and accuracy, which provided a new mode of polyphenol determination in oil -tea camellia seed oil.