文献类型：期刊文献

中文题名：高效液相色谱-质谱联用技术测定毛榛中的紫杉醇含量

英文题名：Simultaneous Determination of Paclitaxel in Hazelnut by HPLC-MS/MS

作者：罗凡[1] 费学谦[1] 汤富彬[1] 李辛雷[1]

第一作者：罗凡

机构：[1]中国林业科学研究院亚热带林业研究所

年份：2011

卷号：24

期号：6

起止页码：779-783

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：中国林科院亚热带林业研究所所长基金(RISF6921);浙江省省院合作林业科技项目(2007SY10)

语种：中文

中文关键词：高效液相色谱-质谱;紫杉醇;毛榛

外文关键词：HPLC-MS/MS paciltaxel hazelnut

分类号：S718.43

摘要：1963年美国化学家M.C.Wani和M.E.Wall首次从一种生长在美国西部森林中称为太平洋紫杉（Pacific yew）的树皮和木材中分离到了紫杉醇粗提物。筛选实验发现,紫杉醇粗提物对离体培养的鼠肿瘤细胞有很高的活性。
Established method by reverse phase HPLC and electrospray ionization mass spectrometry（HPLC-ESI-MS） analysis and hazelnut extract trace of taxol.Through the research,seven mass spectrum characteristics of taxol compounds were contested,and the taxanes database was initially established.It was found that the paciltaxel contents were 23.65 μg·mL-1 and 4.09 μg·mL-1 in leaves and stems respectively.The paciltaxel contents in different parts of hazelnut was slightly different,which were 0.61,0.23 and 0.35 μg·mL-1 in hazel branch,leaf and leather.With these data it can quickly identify the known paclitaxel compounds in hazelnut extract,and can perform the unknown structure analysis to certain degrees.This analysis method is confirmed sensitive,reliable and fast.