文献类型：期刊文献

英文题名：Expression patterns of some genes involved in tanshinone biosynthesis in Salvia miltiorrhiza roots

作者：Yang, Yanfang[1,2,3] Hou, Shuang[1,2] Fan, Wei[4] Lu, Lilan[5,6] Hui, Nan[3] Wu, Xia[7] Wei, Jianhe[1,2]

第一作者：Yang, Yanfang;杨艳芳

通信作者：Wei, JH[1];Wei, JH[2]

机构：[1]Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100193, Peoples R China;[2]Peking Union Med Coll, Beijing 100193, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat State Forestry A, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[4]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[5]Chinese Acad Med Sci, Inst Med Plant Dev, Hainan Branch, Haikou 570311, Hainan, Peoples R China;[6]Peking Union Med Coll, Haikou 570311, Hainan, Peoples R China;[7]Capital Med Univ, Sch Tradit Chinese Med, Beijing 100069, Peoples R China

年份：2019

卷号：130

起止页码：606-614

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000458942600069)】；

基金：This work was supported by the Special Funds in Basic Scientific Research for Non-Profit Research Institutes financed by the Ministry of Finance, People's Republic of China (No. YZ-08-19), National Natural Science Foundation of China (31570675), and a grant for National non-profit Research Institutions of Chinese Academy of Forestry (CAFYBB2018SY009).

语种：英文

外文关键词：Salvia miltiorrhiza; Tanshinone; MEP pathway; MVA pathway; Gene expression

摘要：Tanshinones are the major bioactive components of Salvia miltiorrhiza, and synthesized through the cytosol-localized mevalonic acid (MVA) and plastid-localized methylerythritol 4-phosphate (MEP) pathways. To reveal correlations between gene expression and tanshinone accumulation, transcript-level variations in five candidate genes involved in tanshinone biosynthesis in the roots of S. miltiorrhiza were investigated at different developmental stages. Additionally, the accumulation of tanshinone I, tanshinone IIA, and cryptotanshinone were analyzed by liquid chromatography tandem mass spectrometry. Compared with the other genes examined, SmCMK (encoding the enzyme 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase) expression was significantly positively correlated with tanshinone production, and SmDXR (1-deoxy-D-xylulose 5-phosphate reductoisomerase) likely functions as a non-rate-limiting enzyme in the tanshinone-biosynthesis pathways of S. miltiorrhiza during different developmental stages, with significant correlations observed between SmDXR and SmCMK expression. Furthermore, no significant correlations were observed between the expression of SmAACT (encoding the enzyme acetyl-CoA acyltransferase), SmHMGR2 (encoding the enzyme 3-hydroxy-3-methylglutaryl-COA reductase), and SmFPPS (encoding the enzyme farnesyl diphosphate synthase), which are involved in the MVA pathway, and tanshinone production during the developmental stages examined, suggesting that the MVA pathway might contribute less to tanshinone accumulation as compared with the MEP pathway. The results in this study indicated that SmCMK might play an important role in their biosynthesis and accumulation. Overall, this study contributes to a better understanding of tanshinone biosynthesis during the different developmental stages of S. miltiorrhiza.