文献类型：期刊文献

英文题名：Identification and characterization of a novel NAC-like gene in chrysanthemum (Dendranthema lavandulifolium)

作者：Yang, Yanfang[1,2] Zhu, Kai[2] Wu, Jian[2] Liu, Liqing[4] Sun, Guiling[5] He, Yanbiao[5] Chen, Fadi[3] Yu, Deyue[2]

第一作者：杨艳芳;Yang, Yanfang

通信作者：Yu, DY[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Nanjing Agr Univ, Natl Ctr Soybean Improvement, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China;[3]Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China;[4]Fujian Inst Subtrop Bot, Fujian Prov Key Lab Subtrop Plant Physiol & Bioch, Xiamen 361009, Peoples R China;[5]Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Peoples R China

年份：2016

卷号：35

期号：8

起止页码：1783-1798

外文期刊名：PLANT CELL REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000379851100016)】；

语种：英文

外文关键词：Transcription factor; NAC; Abiotic stress; Chrysanthemum

摘要：A NAC -like gene named DlNAC1 was identified in chrysanthemum and characterized; it may be involved in regulation of response to abiotic stressors, especially in tolerance to drought and salinity. NAC transcription factors in plants play crucial roles in tolerance to abiotic stressors, and overexpression of the NAC gene in Arabidopsis has been demonstrated to lead to improved drought tolerance. Functions of the NAC genes in chrysanthemum, however, remain poorly understood. In this study, a NAC-like gene named DlNAC1 was identified in chrysanthemum (Dendranthema lavandulifolium) and characterized. Phylogenetic analysis indicated that DlNAC1 contains a typical NAC domain and belongs to the ONAC022 subgroup. According to the subcellular localization and yeast one-hybrid assay, the DlNAC1 protein is localized to nuclei and has a transcription activation ability. Moreover, quantitative real-time PCR analyses showed that DlNAC1 was induced by low-temperature, high-salinity, and drought conditions (separately), but not by abscisic acid (ABA) and heat shock. In these experiments, the downstream genes of NAC transcription factors were found to be up-regulated, including stress-responsive genes KIN1 and AMY1. To further explore the effects of DlNAC1 in response to abiotic stressors, DlNAC1 was overexpressed in tobacco, and these transgenic plants showed significantly enhanced tolerance to drought and salinity. This study suggests that in chrysanthemum, the DlNAC1 gene is involved in regulation of the response to abiotic stressors, especially in tolerance to drought and salinity.