文献类型：期刊文献

中文题名：不同纯度靛蓝提取物的制备及其抑菌活性研究

英文题名：Preparation of Different Purity Indigo Extracts and Study of Their Antibacterial Activities

作者：颜洋洋[1] 张昌伟[1,2] 李川[1] 周昊[1] 王成章[1,2]

第一作者：颜洋洋

机构：[1]中国林业科学研究院林产化学工业研究所,江苏省生物质能源与材料重点实验室,国家林业和草原局林产化学工程重点实验室,林木生物质低碳高效利用国家工程研究中心,江苏省林业资源高效加工利用协同创新中心,江苏南京210042;[2]中国林业科学研究院林业新技术研究所,北京100091

年份：2023

卷号：43

期号：1

起止页码：104-112

中文期刊名：林产化学与工业

外文期刊名：Chemistry and Industry of Forest Products

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：中国林科院中央级公益性科研院所基本科研业务费专项资金(CAFYBB2021ZC003)。

语种：中文

中文关键词：马蓝;靛蓝;纯化;葡萄糖还原;响应面法

外文关键词：Baphicacanthus cusia(Nees)Bremek;indigo;purification;glucose reduction;response surface method

分类号：TQ35

摘要：在建立靛蓝含量分析方法的基础上,构建了一种新型高效方法制备高纯度靛蓝,并研究了不同靛蓝粗提物和高纯度靛蓝的抑菌活性。以马蓝叶为原料,通过水提取、加碱打花、酸中和以及氢氧化钠除杂等步骤得到了3种不同靛蓝质量分数(3.06%、20.13%、22.16%)粗提物样品1~样品3;在此基础上,利用单因素试验和Box-Behnken响应面设计法优化葡萄糖还原工艺得到了高纯度靛蓝样品4;最后,采用牛津杯法比较了3种靛蓝粗提物和高纯度靛蓝对黑曲霉、金黄色葡萄球菌和大肠杆菌的抑菌活性。结果表明:葡萄糖还原工艺最佳条件为葡萄糖质量浓度25 g/L、NaOH质量浓度26.9 g/L、反应温度60℃和反应时间30 min;在此最佳工艺条件下,可得到纯度为96.87%的高纯度靛蓝(样品4),并且靛蓝收率高达76.88%。抑菌实验结果表明:3种靛蓝粗提物和高纯度靛蓝均对黑曲霉、金黄色葡萄球菌和大肠杆菌具有抑制效果,且随着样品中靛蓝质量分数的增加,样品对3种菌的抑制效果均增加;其抑菌圈直径、最小抑菌浓度(MIC)、最小杀菌浓度(MBC)和半数抑菌浓度(IC_(50))分别为9.00~19.69 mm、1~512 mg/L、8~64 mg/L(黑曲霉)和0.6~448 mg/L,高纯度靛蓝对黑曲霉的抑制作用良好,其MIC、MBC和IC_(50)值分别为1、8和0.6 mg/L。
On the basis of establishing the analysis method of indigo content,a new and efficient method for the preparation of indigo with high purity was established,and the antibacterial activities of different crude indigo extracts and high purity indigo were studied.Using Baphicacanthus cusia leaves as raw material,three crude extracts samples 1-3 with different indigo mass fractions(3.06%,20.13%,22.16%)were obtained by water extraction,stir with alkali,acid neutralization and sodium hydroxide for impurity removal.On this basis,single factor test and Box-Behnken response surface design method were used to optimize glucose reduction process for acquiring high purity of indigo sample 4.Finally,oxford cup method was employed to compare the antibacterial activities of three kinds of crude indigo extracts and high purity indigo against Aspergillus niger,Staphylococcus aureus and Escherichia coli.Results showed that the optimal conditions of glucose reduction were glucose concentration of 25 g/L,sodium hydroxide concentration of 26.9 g/L,reaction temperature of 60℃and reaction time of 30 min.Under the optimum conditions,the purity of indigo could reach 96.87%(sample 4),and the yield of indigo was 76.88%.Results of bacteriostatic experiment demonstrated that the three kinds of indigo crude extracts and high purity indigo had inhibitory effect on A.niger,S.aureus and E.coli,and the inhibitory effects on the three kinds of bacteria increased with the increase of the mass fraction of indigo in the samples.Moreover,the inhibition zone diameter,MBC values,MIC values and IC_(50)values of these extracts were 9.00-19.69 mm,1-512 mg/L,8-64 mg/L(A.niger)and 0.6-448 mg/L,respectively.The high purity indigo had a good inhibitory effect on A.niger,and its MIC,MBC and IC_(50)values were 1,8 and 0.6 mg/L,respectively.