文献类型：期刊文献

英文题名：Elite Genotype Characterization and Genetic Structure Analysis of the Medicinal Tree Archidendron clypearia (Jack) I. C. Nielsen Using Microsatellite Markers

作者：Li, Dandan[1,2] Gan, Siming[1,2] Weng, Qijie[2] Zhou, Mingming[1,2] Zhou, Changpin[2] Li, Mei[1,2]

第一作者：Li, Dandan

通信作者：Li, M[1];Li, M[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Xiangshan Rd, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, Key Lab Natl Forestry & Grassland Adm Trop Forestr, 682 Guangshan Yi Rd, Guangzhou 510520, Peoples R China

年份：2024

卷号：15

期号：7

外文期刊名：FORESTS

收录：;EI(收录号：20243116780948);Scopus(收录号：2-s2.0-85199620175);WOS:【SCI-EXPANDED(收录号:WOS:001278801000001)】；

基金：This work was funded by the Fundamental Research Funds of the Chinese Academy of Forestry (grant number CAFYBB2018SY019) and the Public Welfare Research and Capacity Building Funds for Social Development from the Department of Science and Technology of Guangdong Province, China (grant number 2017A020213002).

语种：英文

外文关键词：Archidendron clypearia; medicinal tree; microsatellite marker; elite genotype; fingerprinting; genetic structure

摘要：Archidendron clypearia (Jack) I. C. Nielsen is an important tree species for traditional medicine purposes such as anti-inflammatory, detoxification, uric acid control and neuro-protection. Here, a set of 15 microsatellite markers were used to fingerprint 248 elite genotypes from six origins of A. clypearia and investigate the genetic structure of these genotypes. A total of 170 alleles were amplified. The elite genotypes showed moderate genetic variability, with mean observed heterozygosity (Ho) and expected heterozygosity (He) being 0.651 and 0.699, respectively. Each of the elite genotypes could be fingerprinted uniquely. The cumulative probability of identity (PI) and the cumulative probability of paternity exclusion (PE) were 2.25 x 10(-12) and 0.999992, respectively, demonstrating the extremely high power of the markers for genotype identification. In STRUCTURE and clustering analyses, the same origin of elite genotypes tended to be grouped in a sub-population and clustered closely, but some genotypes from different geographic origins were mixed, suggesting a low level of genetic differentiation between the origins. These results can contribute to the protection and management of the elite genotype resources of A. clypearia and the comprehensive understanding of its genetic structure.