文献类型：期刊文献

中文题名：白蜡虫脂酰辅酶A还原酶基因家族鉴定及序列分析

英文题名：Identification and Sequence Analyses of Fatty acyl-CoA Reductase Gene Family of the Chinese White Wax Scale Insect(Ericerus pela)

作者：柳伟[1] 安佳琪[1] 高熹[2] 杨璞[1,3]

第一作者：柳伟

机构：[1]中国林业科学研究院高原林业研究所,云南昆明650224;[2]云南农业大学植物保护学院,云南昆明650201;[3]国家林业和草原局,资源昆虫培育与利用重点实验室,云南昆明650224

年份：2022

卷号：37

期号：5

起止页码：743-752

中文期刊名：云南农业大学学报：自然科学版

外文期刊名：Journal of Yunnan Agricultural University:Natural Science

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：中央级公益性科研院所基本科研业务费专项(CAFYBB2017ZB005);云南省科技厅基础研究专项计划(202101AS070042);云南省中青年学术和技术带头人后备人才项目(202105AC160031);国家自然科学基金项目(31572337)。

语种：中文

中文关键词：白蜡虫;脂酰辅酶A还原酶;基因家族;结构域;系统进化

外文关键词：Chinese white wax scale insect;fatty acyl-CoA reductase;gene family;domain;phylogenetic analysis

分类号：S899.1

摘要：【目的】对白蜡虫脂酰辅酶A还原酶(fatty acyl-CoA reductase,far)基因家族进行鉴定和序列分析。【方法】以泌蜡昆虫白蜡虫作为材料,利用生物信息学分析方法对白蜡虫far基因家族进行鉴定。【结果】在白蜡虫基因组中共鉴定到30个far候选基因,其中22个FAR结构域包含完整的1个NAD(P)H结合结构域和1个FAR C结构域。基序分析表明:多数白蜡虫FAR含有13个保守基序。白蜡虫far基因有6~11个外显子、5~10个内含子。白蜡虫FAR系统进化分析表明:白蜡虫FAR成员聚为2类。对不同物种FAR进行的系统进化分析表明:FAR聚类主要与物种亲缘关系有关,与功能的相关性不大。共线性分析与氨基酸相似性分析显示:亲缘关系相近的物种FAR相似性更高,一般同物种内FAR相似性最高。【结论】本研究有助于了解白蜡虫far基因家族的进化,为深入研究白蜡虫far基因家族参与不同的功能奠定基础。
[Purpose]To identify the fatty acyl-CoA reductase(far)gene family and to analyze the gene sequences.[Method]The far gene family of the Chinses white wax scale insect(Ericerus pela)was identified and analyzed by bioinformatics methods.[Results]There were a total of 30 candidate far genes were identified,and 22 genes of them had a relatively conserved structure,an NAD(P)H binding domain and a FAR C domain.Motif analysis showed that there were 13 conserved motifs in these FAR.The far genes of E.pela had 6-11exons and 5-10 introns.The phylogenetic ana-lysis showed that FAR of E.pela were divided into two groups.The phylogenetic tree based on FAR of different species revealed FAR cluster groups closely related to species rather than their functions.Synteny analysis and amino acid similarity analysis showed that FAR generally had the highest simil-arity within the same species.[Conclusion]This study was helpful to understand the evolution of far gene family in E.pela and provided a basis for further research on the molecular functions of far gene family of E.pela.