文献类型：期刊文献

英文题名：Optimization Technology of the LHS-1 Strain for Degrading Gallnut Water Extract and Appraisal of Benzene Ring Derivatives from Fermented Gallnut Water Extract Pyrolysis by Py-GC/MS

作者：Wang, Chengzhang[1,2,3,4,5] Li, Wenjun[1,2,3,4,5]

第一作者：王成章;Wang, Chengzhang

通信作者：Wang, CZ[1];Wang, CZ[2];Wang, CZ[3];Wang, CZ[4];Wang, CZ[5]

机构：[1]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Nanjing 210042, Jiangsu, Peoples R China;[2]Natl Engn Lab Biomass Chem Utilizat, Nanjing 210042, Jiangsu, Peoples R China;[3]State Forestry Adm, Key & Open Lab Forest Chem Engn, Nanjing 210042, Jiangsu, Peoples R China;[4]Key Lab Biomass Energy & Mat, Nanjing 210042, Jiangsu, Peoples R China;[5]Chinese Acad Forestry, Inst New Technol Forestry, Beijing 100091, Peoples R China

年份：2017

卷号：22

期号：12

外文期刊名：MOLECULES

收录：;Scopus(收录号：2-s2.0-85040337942);WOS:【SCI-EXPANDED(收录号:WOS:000419242400215)】；

基金：This work was supported by the work was supported by the National Key Research & Development Program (No. 2016YFD0600805) and National Natural Science Foundation of China (No. 31570564).

语种：英文

外文关键词：gallnut water extract; tannic acid; benzene ring derivatives; biodegradation; Py-GC; MS

摘要：Gallnut water extract (GWE) enriches 80 similar to 90% of gallnut tannic acid (TA). In order to study the biodegradation of GWE into gallic acid (GA), the LHS-1 strain, a variant of Aspergillus niger, was chosen to determine the optimal degradation parameters for maximum production of GA by the response surface method. Pyrolysis-gas chromatography-mass spectrometry (Py-GC/MS) was first applied to appraise benzene ring derivatives of fermented GWE (FGWE) pyrolysis by comparison with the pyrolytic products of a tannic acid standard sample (TAS) and GWE. The results showed that optimum conditions were at 31 degrees C and pH of 5, with a 50-h incubation period and 0.1 gL-1 of TA as substrate. The maximum yields of GA and tannase were 63 similar to 65 mgmL-1 and 1.17 UmL-1, respectively. Over 20 kinds of compounds were identified as linear hydrocarbons and benzene ring derivatives based on GA and glucose. The key benzene ring derivatives were 3,4,5-trimethoxybenzoic acid methyl ester, 3-methoxy-1,2-benzenediol, and 4-hydroxy-3,5-dimethoxy-benzoic acid hydrazide.