文献类型：期刊文献

中文题名：茶油水酶法提取工艺初步研究

英文题名：A Preliminary Study on the Extraction Techology of Oil-tea Camellia Seed Oil by Aqueous Enzymatic Method

作者：孙红[1] 费学谦[1] 方学智[1]

第一作者：孙红

机构：[1]中国林科院亚热带林业科学研究所

年份：2011

卷号：33

期号：3

起止页码：597-601

中文期刊名：江西农业大学学报

外文期刊名：Acta Agriculturae Universitatis Jiangxiensis

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：国家"十一五"科技支撑计划项目(2009BADB1B03);农业部"948"引进项目(2009-4-56)

语种：中文

中文关键词：茶油;水酶法;酶筛选;提油率

外文关键词：oil-tea camellia seed oil; aqueous enzymatic method; enzymatic selection; oil yield

分类号：TS225.16

摘要：采用水酶法提油技术从油茶籽中提取茶油,研究酶的种类及不同的多酶组合对提油率的影响,并筛选茶油水酶法提取的最佳水解酶和酶组合。结果显示,单一酶中Alcalase 2.4 L蛋白酶具有较好的提油效果,提油率达到90.48%,而通过多级筛选试验获得的最佳多酶组合方案为依序使用果胶酶、纤维素酶和Alcalase 2.4 L蛋白酶对料液进行酶解,提油率可达91.40%。综合分析,在茶油的水酶法提取中选用Alcalase 2.4 L蛋白酶作为水解酶是适宜的选择。
An aqueous enzymatic extraction technology was adopted to extract oil from Oil-tea camellia seed.The influences of different enzymes and enzyme complex formulas on oil yield were investigated.The result showed that Alcalase 2.4 L was the best single enzyme with the highest oil yield of 90.48%.The best enzyme complex formula was composed by pectinase,cellulose and Alcalase 2.4 L,which was gained by multilevel optimization procedure.The oil yield efficiency could reach 91.40% when this enzyme complex formula was used in sequence.By comprehensive consideration,Alcalase 2.4 L is the best hydrolase.