文献类型：期刊文献

中文题名：白蜡虫蜡花微生物多样性分析

英文题名：Analysis of the diversity of microorganisms in the wax secreted by the Chinese white wax scale insect,Ericerus pela(Chavannes)(Hemiptera:Coccidae)

作者：王雪庆[1] 于淑惠[1] 孙涛[1] 赵遵岭[1] 陈晓鸣[1] 杨璞[1]

第一作者：王雪庆

机构：[1]中国林业科学研究院资源昆虫研究所,国家林业局资源昆虫培育与利用重点实验室,昆明650224

年份：2016

卷号：59

期号：10

起止页码：1086-1092

中文期刊名：昆虫学报

外文期刊名：Acta Entomologica Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家“863”计划项目(2014AA021801);林业公益性行业科研专项(201504302;201304808);云南省应用基础研究重点项目(2013FA052);国家自然科学基金项目(31572337)

语种：中文

中文关键词：白蜡虫;蜡花;细菌;真菌;群落组成;物种多样性

外文关键词：Ericerus pela; wax; bacteria; fungi; community composition; species diversity;

分类号：Q965.8

摘要：【目的】探究白蜡虫Ericerus pela(Chavannes)雄若虫分泌的蜡花中微生物的多样性,比较不同厚度蜡花(随雄若虫发育蜡质层厚度增加)中细菌及真菌的种类和变化。【方法】采用Mi Seq高通量测序方法对不同发育阶段白蜡虫雄若虫分泌的蜡花中细菌16S rRNA和真菌ITS进行测序,利用Mothur软件计算蜡花中细菌和真菌的操作分类单元(operational taxonomic unit,OTU)及其丰富度、Alpha多样性分析。【结果】细菌3个样品共获470个OTU,真菌2个样品共获264个OTU。细菌共鉴定出18门30纲92科173属;真菌共鉴定出3门15纲43科68属。其中丛毛单胞菌属Comamonas细菌在蜡花中为优势菌,分别占前、中、后期雄若虫分泌的蜡花中细菌总量的79.79%,41.61%和46.86%;枝胞属Cladosporium在前期雄若虫分泌的蜡花中占真菌总量的17.01%,隐球菌属Cryptococcus在后期雄若虫分泌的蜡花中占真菌总量的60.84%,是蜡花中真菌重要组成部分。【结论】不同发育阶段雄若虫分泌的蜡花中细菌及真菌群落存在差异,随着蜡花厚度增加,细菌多样性先增加后减少,而真菌多样性降低。
【Aim】This study aims to explore the diversity of microorganisms in the wax secreted by male nymphs of Ericerus pela（ Chavannes）,to analyze the bacterial and fungal species and to compare the microorganism change in the wax layers with different thicknesses（ the wax layer thickens with the developmental stage of male nymphs）. 【Methods】The Mi Seq high-throughput sequencing method was employed to sequence the 16 S rRNA of bacteria and the internal transcribed space（ ITS） of fungi in the wax secreted by male nymphs of E. pela at different developmental stages. The bacterial and fungal OTUs,richness and alpha diversity was calculated and analyzed with the Mothur software. 【Results】According to 470 OTUs obtained from 3 bacterial samples,18 phyla,30 classes,92 families and 173 genera were identified. Meanwhile,3 phyla,15 classes,43 families and 68 genera were identified from264 OTUs obtained from 2 fungal samples. As the dominant bacteria,Comamonas accounted for 79. 79%,41. 61% and 46. 86% of the total bacteria in the wax secreted by male nymphs at the early,middle and late stages,respectively. Cladosporium and Cryptococcus were two important components of fungi in the wax,accounting for 17. 01% and 60. 84% fungi in the wax secreted by male nymphs at the early and late stages,respectively. 【Conclusion】There are some differences in bacterial and fungal communities in the wax secreted by male nymphs of E. pela at different developmental stages. The bacterial diversity in the wax layers increases initially and then decreases with the developmental stage of male nymph,while the fungal diversity in the wax layers decreases with the wax thickening.