文献类型：期刊文献

英文题名：Preparation of Ginkgo biloba seed protein hydrolysate-zinc chelate and investigation of its adsorption mechanism on insoluble dietary fiber: kinetic, isotherm and thermodynamic characterizations

作者：Deng, Yejun[1] Lu, Yi[1] Wang, Xiang[1] Zhang, Caihong[1] Xie, Pujun[1] Huang, Lixin[1,2,3]

第一作者：邓叶俊

通信作者：Huang, LX[1]

机构：[1]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Natl Key Lab Dev & Utilizat Forest Food Resources, Nanjing 210042, Peoples R China;[2]Natl Forestry & Grassland Adm, Natl Engn Res Ctr Low Carbon Proc & Utilizat Fores, Key Lab Biomass Energy & Mat, Key Lab Chem Engn Forest Prod, Nanjing 210042, Jiangsu, Peoples R China;[3]Nanjing Forestry Univ, Int Innovat Ctr Forest Chem & Mat, Jiangsu Coinnovat Ctr Efficient Proc & Utilizat Fo, Nanjing 210042, Peoples R China

年份：2026

卷号：411

外文期刊名：JOURNAL OF FOOD ENGINEERING

收录：;EI(收录号：20260319911926);Scopus(收录号：2-s2.0-105027331066);WOS:【SCI-EXPANDED(收录号:WOS:001659559900001)】；

基金：This work was supported by Central Public Welfare Research In-stitutes'Special Fund for Basic Research Operation Costs [grant number: CAFYBB2023MB027] , National Natural Science Foundation of China [grant number: 32171732] and Jiangsu Key Laboratory of Biomass Energy and Materials [grant number: JSBEM-S-202306] .

语种：英文

外文关键词：Ginkgo biloba; Peptide-zinc chelate; In vitro stability; Adsorption feature; Dietary zinc supplement

摘要：The instability of zinc in the gastrointestinal tract and its adsorption on insoluble dietary fibers (IDF) are significant factors contributing to zinc deficiency. To address this, Ginkgo biloba seed protein isolate hydrolysate (GPIH) was enzymatically prepared and chelated with ZnSO47H(2)O to obtain GPIH-Zn. Single-factor optimization determined the optimal chelation conditions: pH 6.0, GPIH/ZnSO47H(2)O mass ratio of 1:1, temperature 50 degrees C, and chelation time 30 min, yielding a zinc chelating capacity of 135.03 mg/g. The results of ultraviolet-visible absorption and fluorescence spectroscopy synthetically confirmed the chelation between GPIH and Zn, and Fourier-transform infrared spectroscopy revealed that the -NH2, COO-, -N-H, C=O, C-H, and -OH groups primarily participated in chelating. The GPIH-Zn chelate was more soluble at various pH, and presented excellent stability in simulated gastrointestinal environment, which could promote zinc absorption in human gastrointestinal tract. The adsorption features of GPIH-Zn chelate on carrot IDF (CIDF) were investigated. Compared to free zinc ions, the zinc in GPIH-Zn chelate showed a >10 % reduction in maximum adsorption capacity on CIDF. The results showed Freundlich isotherm model and pseudo-second-order kinetics model yielded the best fit to adsorption data, implying multi-layer adsorption of GPIH-Zn on CIDF, and it was a chemisorption. Thermodynamically, zinc ions adsorption onto CIDF was endothermic, whereas GPIH-Zn adsorption was a spontaneous and exothermic reaction. This work assisted understanding of the adsorption mechanism of GPIH-Zn chelate on to IDF. These findings demonstrate the potential of GPIH-zinc chelate as an effective zinc supplement with high stability and capacity to combat dietary fibers' adsorption.