文献类型：期刊文献

中文题名：基于代谢组学法鉴定液体果蜡中漂白紫胶的模型构建

英文题名：Metabolomics-Based Identification of Bleached Shellac in Liquid Fruit Wax

作者：李坤[1,2] 张雯雯[1,2] 唐保山[2] 邢淑婕[1] 马金菊[2] 朱静[1] 雷福厚[3] 张弘[2]

第一作者：李坤

机构：[1]信阳农林学院食品学院,河南信阳464000;[2]中国林业科学研究院高原林业研究所,国家林业和草原局特色森林资源工程技术研究中心,云南昆明650233;[3]广西民族大学广西林产化学与工程重点实验室,广西林产化学与工程协同创新中心,广西南宁530006

年份：2023

卷号：44

期号：14

起止页码：377-383

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD;;EI(收录号：20233914782618);Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：信阳农林学院青年基金项目(QN2021030);信阳农林学院科技创新团队项目(XNKJTD-001);广西民族大学开放基金项目(GXFG2012);河南省科技攻关项目(232102110163);河南省高等学校重点科研项目(23A550015)。

语种：中文

中文关键词：漂白紫胶;鉴定;液体果蜡;液相色谱-四极杆串联飞行时间质谱

外文关键词：bleached shellac;authentication;liquid fruit wax;liquid chromatography-quadrupole time-of-flight mass spectrometry

分类号：Q599;S713;TQ917

摘要：为鉴定液体果蜡中是否含有漂白紫胶,借鉴代谢组学的研究思路和方法,采用液相色谱-串联四极杆飞行时间质谱定性,并结合液相色谱蒸发光散射检测器对不同来源的漂白紫胶进行靶向化合物分析。通过无监督的主成分分析和有监督的偏最小二乘判别分析等多元统计分析,筛选出12种靶向化合物。根据变量投影重要性值分为5种差异性化合物和7种共性化合物。最后,以共性化合物为生物标识物,在市售的商业化液体果蜡产品中,验证了该方法的可行性,通过该研究结论并结合色谱-质谱联用技术可以鉴定液体果蜡中是否含有漂白紫胶。
The purpose of this study was to identify whether liquid fruit waxes could be added with bleached shellac.The compounds of bleached shellac from different sources were analyzed by targeted metabolomics based on liquid chromatography-quadrupole time-of-flight mass spectrometry(LC-QTOF-MS)combined with liquid chromatography with evaporative light scattering detection.Twelve target compounds were selected by multivariate statistical analysis including unsupervised principal component analysis(PCA)and supervised partial least squares discriminant analysis(PLS-DA).According to variable importance in the projection(VIP)value,five differential compounds and seven shared compounds were identified.Finally,the viability of this method in the detection of commercial liquid fruit wax products was verified using the shared compounds as biomarkers.These results showed that chromatography combined with mass spectrometry can identify whether bleached shellac could be added in liquid fruit wax.