文献类型：期刊文献

英文题名：A novel copper-based fungicide with enhanced hydrophobic leaf deposition and antimicrobial activities

作者：Liu, Yong[1] Deng, Yejun[1,2] Xie, Pujun[1,2] Wang, Xiang[1,2] Zhang, Caihong[1,2] Huang, Lixin[1,2,3]

通信作者：Huang, LX[1]

机构：[1]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Natl Key Lab Dev & Utilizat Forest Food Resources, Nanjing 210042, Peoples R China;[2]Natl Forestry & Grassland Adm, Natl Engn Res Ctr Low Carbon Proc & Utilizat Fores, Key Lab Biomass Energy & Mat, Key Lab Chem Engn Forest Prod, Nanjing 210042, Peoples R China;[3]Nanjing Forestry Univ, Int Innovat Ctr Forest Chem & Mat, Jiangsu Coinnovat Ctr Efficient Proc & Utilizat Fo, Nanjing 210042, Peoples R China

年份：2026

卷号：732

外文期刊名：COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS

收录：;EI(收录号：20255019676134);Scopus(收录号：2-s2.0-105023971742);WOS:【SCI-EXPANDED(收录号:WOS:001638651600007)】；

基金：This work was supported by the National Key Research and Devel-opment Program of China [grant number: 2022YFD2200804] .

语种：英文

外文关键词：Gleditsia sinensis Lam. saponin-copper chelate; Saponin-metal chelation; Citrus canker control; Eco-friendly agrochemical; Antibacterial efficacy; Enhanced deposition and rainfastness

摘要：While inorganic copper-based fungicides effectively control citrus canker, their poor leaf adhesion and deposition lead to significant waste and copper pollution. To address this issue, we developed a novel, natural, and efficient antibacterial agent, Gleditsia sinensis Lam. saponin-copper chelate (GSS-Cu). The physicochemical properties of GSS-Cu were characterized by EDS, FTIR, XRD, TG, DSC, and pH stability tests. Results revealed that GSS primarily chelates Cu2 + via hydroxyl groups, forming an amorphous complex with enhanced stability in alkaline conditions (85.16 +/- 2.35 % solubility at pH 10 compared to 0.70 +/- 0.084 % for CuSO4). Molecular docking further revealed hydrogen bonding between saponin and Cu2+, providing the first evidence for metal ion chelation by saponins. GSS-Cu also exhibited good wettability on citrus leaves, with a contact angle of 45.63 degrees +/- 0.29 degrees, significantly lower than CuSO4 (80.67 degrees +/- 0.51 degrees) and Bordeaux mixture (BM) (85.64 degrees +/- 0.58 degrees). GSS-Cu achieved higher copper deposition (18.40 +/- 1.63 mu g/cm(2)) and rainfastness (43.10 +/- 2.59 %) than CuSO4 (10.83 +/- 0.92 mu g/cm(2), 20.61 +/- 2.50 %) and BM (17.34 +/- 0.98 mu g/cm(2), 32.05 +/- 4.31 %), attributed to its ability to wet hydrophobic citrus leaves effectively and form a durable, rainfastness protective layer. In antibacterial tests against Xanthomonas citri subsp. citri, GSS-Cu showed an EC50 of 31.54 +/- 6.11 mu g/mL, 3.4 and 4.0 times more potent than CuSO4 (106.46 +/- 14.43 mu g/mL) and BM (124.92 +/- 1.55 mu g/mL), respectively. Its efficacy is linked to the induction of reactive oxygen species. Additionally, GSS-Cu caused no phytotoxicity to citrus leaves and was rapidly adsorbed by soil (>75 % within 4 h), indicating favorable environmental compatibility. With its lower copper usage, good wettability, adhesion, antimicrobial activity, and eco-friendly profile, GSS-Cu represents a promising agrochemical.