文献类型：期刊文献

中文题名：皂荚皂素-银离子螯合物的构建及其性质研究

英文题名：Construction and Properties of Gleditsia sinensis Saponin-silver Ion Chelates

作者：邓叶俊[1] 黄立新[1] 张彩虹[1] 谢普军[1]

第一作者：邓叶俊

机构：[1]中国林业科学研究院林产化学工业研究所,江苏省生物质能源与材料重点实验室,国家林业和草原局林产化学工程重点实验室,林木生物质低碳高效利用国家工程研究中心,江苏省林业资源高效加工利用协同创新中心,江苏南京210042

年份：2025

卷号：45

期号：5

起止页码：1-8

中文期刊名：林产化学与工业

外文期刊名：Chemistry and Industry of Forest Products

收录：;北大核心:【北大核心2023】；

基金：国家重点研发计划资助项目(2022YFD2200804-3)。

语种：中文

中文关键词：皂素;泡沫稳定性;抑菌

外文关键词：saponin;foam stability;antibacterial

分类号：TQ35;TS202.1

摘要：为强化皂荚皂素(GSS)的抑菌活性,制备了皂荚皂素-银离子螯合物(GSS-Ag)。通过单因素试验确定了较佳的制备工艺,利用红外光谱(FT-IR)、扫描电镜(SEM)、能谱仪(EDS)和纳米激光粒度仪等对GSS-Ag的理化性质进行了表征,分析了GSS-Ag的发泡能力和泡沫稳定性,并研究了GSS-Ag的抑菌活性和机理。研究结果表明:GSS-Ag较佳的制备工艺为pH值7、温度50℃和m(GSS)∶m(AgNO3)=10∶1,在该条件下GSS对Ag^(+)的螯合能力达7.56 mg/g。理化性质测试结果发现:GSS中—OH参与了对Ag^(+)的螯合,螯合过程没有改变GSS的基本化学结构;螯合后Ag^(+)在GSS中分布均匀,形成了稳定的螯合物;GSS-Ag平均粒径为17.94 nm,Zeta电位为-6.32 mV,相比GSS的平均粒径36.70 nm、Zeta电位-22.0 mV,均发生明显变化,说明Ag^(+)与GSS中带负电的官能团(—OH)发生了螯合反应。GSS-Ag泡沫性能优异,在低质量分数(0.001%)下发泡能力为69.3%,是吐温80的1.5倍;且泡沫稳定性良好,240 min时GSS-Ag的泡沫稳定性为61.95%,高于吐温80的51.49%。此外,GSS-Ag对大肠杆菌和金黄色葡萄球菌的最低抑菌质量浓度(MIC)分别为2.5和0.625 g/L,相比GSS的MIC值(均>100 g/L)明显降低;碱性磷酸酶(AKP)活力测定结果表明:GSS-Ag可通过破坏细菌细胞壁发挥抑菌作用。
In order to enhance the antibacterial activity of Gleditsia sinensis saponin(GSS),the GSS-silver iron chelate was prepared.The optimal preparation process of GSS-Ag was determined through single-factor experiments.The physicochemical properties of GSS-Ag were characterized using Fourier transform infrared spectrometer(FT-IR),scanning electron microscopy(SEM),energy disperse spectroscopy(EDS),and dynamic light scattering nano-particle size analyzer.The foaming capacity and foam stability of GSS-Ag were analyzed.Meanwhile,the antibacterial activity and mechanism of GSS-Ag were investigated.The results indicated that the optimal preparation conditions for GSS-Ag were as follows:pH value of 7,temperature of 50℃,and the m(GSS)∶m(AgNO3)=10∶1.Under these conditions,the chelation capacity of GSS to Ag^(+)reached 7.56 mg/g.The physicochemical property test results indicated that the hydroxyl groups in GSS participated in chelating Ag^(+)without changing the basic chemical structure of GSS.After chelation,Ag^(+)distributed uniformly in GSS,forming a stable chelate.The average particle size of GSS-Ag was 17.94 nm,and the Zeta potential was-6.32 mV,which underwent significant changes compared to the average particle size of 36.70 nm and the Zeta potential of-22.0 mV in GSS,indicating that Ag^(+)had formed a chelate with the negatively charged functional groups(-OH)in GSS.GSS-Ag exhibited excellent foam performance with a foaming capacity of 69.3%at a low mass fraction(0.001%),which was 1.5 times that of Tween 80.Moreover,GSS-Ag showed a good foam stability.The foam stability of GSS-Ag was 61.95%at 240 min,higher than that of Tween 80(51.49%).In addition,the minimum inhibitory mass concentrations(MIC)of GSS-Ag against Escherichia coli and Staphylococcus aureus were 2.5 and 0.625 g/L,respectively,which was significantly lower than that of GSS(both>100 g/L).The results of alkaline phosphatase(AKP)activity test showed that GSS-Ag could exert antibacterial effects by destroying the bacterial cell wall.