详细信息
Al3+荧光增敏快速检测酸奶和乳酸菌饮料中的胭脂虫红酸(英文) ( EI收录)
Rapid Detection of Carminic Acid in Yogurt and Lactobacillus-Containing Beverage by Al3+-Based Fluorescence Enhancement
文献类型:期刊文献
中文题名:Al3+荧光增敏快速检测酸奶和乳酸菌饮料中的胭脂虫红酸(英文)
英文题名:Rapid Detection of Carminic Acid in Yogurt and Lactobacillus-Containing Beverage by Al3+-Based Fluorescence Enhancement
作者:刘兰香[1] 伊冠东[1,2] 李凯[1] 马金菊[1] 徐涓[1] 孙彦琳[2] 张弘[1]
第一作者:刘兰香
通信作者:Zhang, Hong
机构:[1]中国林业科学研究院资源昆虫研究所,云南昆明650224;[2]昆明理工大学化学工程学院,云南昆明650500
年份:2020
卷号:41
期号:2
起止页码:306-314
中文期刊名:食品科学
外文期刊名:Food Science
收录:CSTPCD;;EI(收录号:20202108693585);Scopus;北大核心:【北大核心2017】;CSCD:【CSCD_E2019_2020】;
基金:“十三五”国家重点研发计划重点专项(2016YFD0600806);中央级公益性科研院所基本科研业务费专项(CAFYBB2017MA012);云南大学化学工程学院重点实验室开放课题(YNFC19-08)
语种:中文
中文关键词:胭脂虫红酸;荧光;增强;快速检测
外文关键词:carminic acid;????uorescence;enhancement;fast detection
分类号:TS202.3;TQ617.1
摘要:研究溶剂、浓度、pH值以及金属离子对胭脂虫红酸荧光性能的影响,表明胭脂虫红酸在pH 4且其物质的量浓度为2.0×10^-4 mol/L的水溶液中荧光最强;而胭脂虫红酸对金属离子选择性识别的结果显示,Al^3+能使胭脂虫红酸的荧光强度显著增强从而提高检测的灵敏度。以Al^3+(2.0×10^-3 mol/L)为标准溶液检测样品中胭脂虫红酸含量时,胭脂虫红酸的物质的量浓度在1.0×10^-5~8.0×10^-5 mol/L范围内与其荧光强度呈良好的线性关系,检测限为5.0×10^-6 mol/L。实际应用结果表明,用荧光法检测不同酸奶和乳酸菌饮料中添加的胭脂虫红酸的含量,样品回收率在96.6%~110.5%之间,相对标准偏差小于5.9%。本研究为快速、有效、定量地检测食品中的胭脂虫红酸提供了一种有效的方法。
The effects of solvents,carminic acid concentration,pH and metal ions on the fluorescence properties of carminic acid were systematically studied.The fluorescent intensity of carminic acid was the strongest at a concentration of 2.0×10^-4 mol/L in an aqueous solution at pH 4.The selectivity of carminic acid to metal ions indicated that the addition of Al3+could significantly enhance the fluorescence intensity of carminic acid thus improving the sensitivity of detection.A 2.0×10^-3 mol/L aqueous solution of Al3+(at)was used as the standard solution.The?uorescence intensity of carminic acid was linear with its concentration ranging from 1.0×10^-5 to 8.0×10^-5 mol/L,and the limit of detection(LOD)was 5.0×10^-6 mol/L.When the fluorescence method was applied to determine carminic acid in yogurts and Lactobacillus-containing beverages,the recoveries ranged from 96.6%to 110.5%,relative standard deviation(RSD)no more than 5.9%.This study provides an effective approach to rapidly and quantitatively detect carminic acid in foods.
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