文献类型：期刊文献

中文题名：低温减压沸腾提取橄榄苦苷的工艺研究

英文题名：Boiling extraction of oleuropein at low temperature and reduced pressure

作者：谢普军[1,2] 黄立新[1,2] 张彩虹[1,2] 游凤[1,2] 王成章[1,2] 周昊[1,2]

第一作者：谢普军

机构：[1]中国林业科学研究院林产化学工业研究所生物质化学利用国家工程实验室国家林业局林产化学工程重点开放性实验室江苏省生物质能源与材料重点实验室,江苏南京210042;[2]中国林业科学研究院林业新技术研究所,北京100091

年份：2012

期号：13

起止页码：1946-1951

中文期刊名：中国中药杂志

外文期刊名：China Journal of Chinese Materia Medica

收录：MEDLINE(收录号：23019876);CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；PubMed;

基金：中央级公益性科研院所基本科研业务费专项(CAF-INT2010K05);国家高技术研究发展计划(863)项目(2011AA100802)

语种：中文

中文关键词：减压;油橄榄叶;橄榄苦苷;沸腾提取

外文关键词：low pressure; olive leaf; oleuropein; boiling extraction

分类号：R284.2

摘要：目的:对油橄榄叶进行低温减压沸腾提取橄榄苦苷以期获得提取的优化工艺。方法:根据单因素实验设计,考察了溶剂种类、温度、时间、料液比、乙醇浓度、真空度、提取次数7个因素对橄榄苦苷提取得率的影响;并在单因素实验基础上,选取了影响橄榄苦苷得率更重要的4个因素,分别为温度、时间、料液比及乙醇浓度,进行L9(34)正交试验,再与传统提取方法进行了对比。结果:通过直观分析及方差分析,4个因素都极显著,减压沸腾提取效果更佳。结论:橄榄苦苷低温减压沸腾提取优化条件为温度60℃,时间20 min,料液比1∶30,乙醇浓度85%,并在此优化条件下橄榄苦苷提取得率为5.90%。与传统提取方法及超声辅助提取相比,减压沸腾提取工艺具有时间短,效率高,并具有良好的应用前景。
Objective： To look for optimum extraction techniques for oleuropein by boiling olive leaves at low temperature and reduced pressure. Method： According to single factor experiment （SFE） design, the effects of seven factors, the impact of seven fac- tors, type of solvent, temperature, time, ratio of material to liquid, ethanol concentration, vacuum degree and extraction times, on ex- traction yield of oleuropein were investigated. Based on the results of SFE, four more important factors, temperature, time, ratio of ma- terial to liquid and ethanol concentration, were selected in L^9 （ 3^4 ） orthogonal experiment （OE） to compare with those extracted with traditional methods. Result： The optimum conditions for boiling extraction of oleuropein at low temperature and reduced pressure were as follows： temperature 60℃ , time 20 min, ratio of material to liquid 1：30 and ethanol concentration 85%. The conditions presented an extraction yield of 5.90%. Conclusion： Compared with traditional extraction methods and the ultrasound assisted extraction meth- od, boiling extraction techniques at low temperature and reduced pressure were so quick and efficient that it has a good application prospect.