文献类型：期刊文献

中文题名：酿酒酵母对双斑蟋生长性能及其肠道细菌的影响

英文题名：Effects of Saccharomyces cerevisiae on Growth Performance and Intestinal Bacterium of Gryllus bimaculatus

作者：杨攀丽[1,2,3] 王凤翔[4] 甘瑾[1,3] 何钊[1,3] 孙龙[1,3] 冯颖[1,3] 王成业[1,3] 赵敏[1,3]

第一作者：杨攀丽

机构：[1]中国林业科学研究院高原林业研究所,云南昆明650224;[2]南京林业大学林学院,江苏南京210037;[3]国家林业和草原局资源昆虫培育与利用重点实验室,云南昆明650224;[4]云南师范大学生命科学学院,云南昆明650500

年份：2023

卷号：38

期号：6

起止页码：948-958

中文期刊名：云南农业大学学报(自然科学版)

外文期刊名：Journal of Yunnan Agricultural University:Natural Science

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD_E2023_2024】；

基金：浙江省中国林业科学研究院省院合作林业科技项目(2019SY02);林草国家创新联盟自筹项目(GLM[2021]24号)。

语种：中文

中文关键词：益生菌;酿酒酵母;双斑蟋;生长指标;肠道细菌

外文关键词：probiotics;Saccharomyces cerevisiae;Gryllus bimaculatus;growth index;intestinal bac-terium

分类号：S432.44

摘要：【目的】探究酿酒酵母(Saccharomyces cerevisiae)对双斑蟋(Gryllus bimaculatus De Geer)生长性能及其肠道细菌的影响。【方法】对比以基础饲料和添加酿酒酵母饲料为食的双斑蟋的生长发育指标,并选出酿酒酵母的最优添加量组;对最优组与对照组个体分别进行肠道细菌分析,对比两组之间的菌群分布特点,找出差异菌群。【结果】(1)饲料中添加不同密度的酿酒酵母均可提高双斑蟋的成虫取食量和平均质量,且添加量为5×10^(4) CFU/g时效果最明显。(2)双斑蟋食用添加酿酒酵母的饲料后,肠道菌群与对照组相比差异显著,拟杆菌门(Bacteroidota)转变为高丰度菌群,推测可能是酿酒酵母有益于该门菌群繁衍。(3)PICRUSt2功能预测表明:试验组双斑蟋肠道内与氨基酸代谢和核苷酸代谢有关的细菌群落相对丰度显著高于对照组,但与信号转导和膜转运相关的细菌群落相对丰度较低。【结论】双斑蟋食用添加酿酒酵母的饲料后,可在一定程度上改善其取食量和平均质量,并提高机体肠道内细菌的α多样性及细菌群落的代谢能力。
[Purpose]To explore the effects of Saccharomyces cerevisiae on the growth perform-ance and intestinal bacterium of Gryllus bimaculatus De Geer.[Method]Compared the growth and development indicators of G.bimaculatus fed on basic feed and feed supplemented with S.ces-cerevisiae,and the optimal addition group was selected.The optimal group and the control group were respectively analyzed for intestinal bacterium,and the distribution characteristics of the flora between the two groups were compared to find out the difference.[Results]1)Adding different densities of S.cerevisiae in the diet could improve the feeding amount and average weight of adult crickets,and the effect was most obvious when adding concentration of 5×10^(4) CFU/g.2)In the group feeding on the diet supplemented with probiotic,Bacteroidota in the intestinal tract of crickets turned into a high abundance of bacteria,which might be attributed to the nutrition from S.cerevisiae.3)The functional prediction of PICRUSt2 indicated that the relative abundance of bacterial communities re-lated to amino acid metabolism and nucleotide metabolism in the gut of the treatment group was signi-ficantly higher than that of the control group,but the relative abundance of bacterial communities re-lated to signal transduction and membrane transport was lower.[Conclusion]After eating the feed supplemented with S.cerevisiae,the growth index of the average body weight and feeding amount of G.bimaculatus can be improved to a certain extent,and the alpha diversity and metabolic capacity of bacterial community in the intestinal tract of the crickets are also increased.