文献类型：期刊文献

中文题名：油橄榄叶提取物的制备及理化性质研究

英文题名：Preparation and Physicochemical Properties of Olive Leaf Extract

作者：王晓杰[1,2,3,4] 黄立新[1,2,3,4] 张彩虹[1,2,3,4,5] 谢普军[1,2,3,4] 邓叶俊[1,2,3,4] 刘璐婕[1,2,3,4]

第一作者：王晓杰

机构：[1]中国林业科学研究院林产化学工业研究所;[2]生物质化学利用国家工程实验室;[3]国家林业和草原局林产化学工程重点实验室;[4]江苏省生物质能源与材料重点实验室,江苏南京210042;[5]南京林业大学江苏省林业资源高效加工利用协同创新中心,江苏南京210037

年份：2019

卷号：39

期号：4

起止页码：83-90

中文期刊名：林产化学与工业

外文期刊名：Chemistry and Industry of Forest Products

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家自然科学基金青年基金项目(31800614)

语种：中文

中文关键词：油橄榄叶;提取;大孔树脂;总多酚;总黄酮;橄榄苦苷

外文关键词：olive leaves;extraction;macroporous resin;total polyphenols;total flavonoids;oleuropein

分类号：TQ35;TS201.1

摘要：对油橄榄叶中总多酚、总黄酮和橄榄苦苷热浸提工艺进行放大实验,考察了乙醇体积分数对活性物质提取效果的影响,并运用单指数衰减函数模型对提取过程进行动力学拟合。研究结果表明:80%乙醇提取效果最佳,提取液中总多酚、总黄酮和橄榄苦苷分别为3.77、2.85和3.03 g/L;单指数衰减函数拟合效果较好, R 值均大于0.9,且随乙醇体积分数的升高,曲线拟合效果越好。在进风温度150 ℃、进料速率 15 mL /min的条件下将脱除乙醇的提取液进行喷雾干燥处理,并运用AB-8大孔树脂对粗提物进行纯化后,总多酚质量分数由 9.71%提高到31.65%,总黄酮质量分数由7.22%提高到10.98%,橄榄苦苷质量分数由9.16%提高到20.38%。对干燥后提取物粉末的含水量、孔隙率、密度、水溶性和吸湿性等理化性质进行测定,结果表明:不同体积分数乙醇提取物粉末含水量、密度和孔隙率相差不大,水提取物具有更好的水溶性(溶解度为98.38%),乙醇提取物的吸湿性均小于5%。
The scale-up experiment of hot reflux extraction of total polyphenols, total flavonoids and oleuropein from olive leaves was studied. The effect of ethanol concentration on the extraction of active substances was investigated, and the related kinetics was also evaluated by ExpDec1 model. The results showed the highest contents of total polyphenols, total flavonoids and oleuropein were obtained when the ethanol concentration was 80% and they were 3.77, 2.85 and 3.03 g/L, respectively. ExpDec1 model had a good fitting effect, and the fitting degree R value was higher than 0.9. The higher ethanol concentration, the better the fitness effect observed. Under the condition of inlet temperature 150 ℃ and feeding rate 15 mL/min, the extracts were dried by spray drying, and crude extracts were purified by AB-8 macroporous resin, the content of total polyphenols increased from 9.71% to 31.65%, the content of total flavonoids increased from 7.22% to 10.98%, and the content of oleuropein increased from 9.16% to 20.38%. The physicochemical properties of the extract powder were measured, such as moisture content, porosity, density, dissolution, moisture absorption, active substance, etc. The results showed that moisture content, density and porosity of different extracts were similar, water extract had better water solubility(98.38%), 80% ethanol extract had higher content of the active substance(the contents of total polyphenols, total flavonoids and oleuropein were 9.71%, 7.22% and 9.16%, respectively), and the contents of total flavonoids and oleuropein were about two times higher than that of other solvents.