文献类型：期刊文献

中文题名：油茶籽仁中油脂的水酶法提取研究

英文题名：Aqueous Enzymatic Extraction of Oil-tea Camellia (Camellia oleifera) Seed Oil

作者：方学智[1,2] 王开良[1] 姚小华[1] 王亚萍[1]

第一作者：方学智

机构：[1]中国林业科学研究院亚热带林业研究所;[2]浙江大学生物化学研究所

年份：2010

卷号：23

期号：5

起止页码：778-781

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家林业局林业公益性行业科研专项项目"油茶精深加工与利用关键技术研究"(200704036);国家林业局"948"项目"油脂酶法制取及副产品高效利用技术引进"(2009-4-56)

语种：中文

中文关键词：油茶;水酶法提取;油茶籽

外文关键词：Camellia oleifera; aqueous enzymatic extraction; oil-tea camellia seed

分类号：S794.4

摘要：An aqueous enzymatic extraction method was used to extract oil from oil-tea camellia seed(Camellia oleifera).The factors influencing the yield rate such as enzyme kind,addition methods,concentration of enzyme,enzymatic hydrolysis time and temperature,and ration of solid to liquid were investigated.The results are as following: The acid protease and cellulase have higher oil yield rate than the other treatments,but there is no significant difference between acid protease and cellulase(P<0.05).By directly adding acid protease to seed slurry of oil-tea camellia seed,more oil yield rate can be gotten than those treatments which added acid protease to ground slurry or filtered ground slurry.By single factor experiment,the optimum conditions of enzymolysis are: the concentration of acid protease,0.1 %(w/w);the enzymolysis temperature and time,55 ℃ and 3 h,respectively;and the ratio of solid to liquid,1∶10.
An aqueous enzymatic extraction method was used to extract oil from oil-tea camellia seed （ Camellia oleif- era）. The factors influencing the yield rate such as enzyme kind, addition methods, concentration of enzyme, enzy- matic hydrolysis time and temperature, and ration of solid to liquid were investigated. The results are as following： The acid protease and cellulase have higher oil yield rate than the other treatments, but there is no significant difference between acid protease and cellulase （P 〈0. 05）. By directly adding acid protease to seed slurry of oil-tea camellia seed, more oil yield rate can be gotten than those treatments which added acid protease to ground slurry or filtered ground slurry. By single factor experiment, the optimum conditions of enzymolysis are： the concentration of acid protease, 0.1% （w/w） ; the enzymolysis temperature and time, 55 ℃ and 3 h, respectively and the ratio of solid to liquid, 1： 10.