文献类型：期刊文献

英文题名：Widely targeted metabolomic and KEGG analyses of natural deep eutectic solvent-based saponins extraction from Camellia oleifera Abel.: Effects on composition

作者：Wei, Zhenqian[1] Zhang, Weiyan[1,2] Du, Menghao[1] Zhong, Haiyan[2] Fang, Xuezhi[1]

第一作者：Wei, Zhenqian

通信作者：Fang, XZ[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, 73 Daqiao Rd, Hangzhou 311400, Zhejiang, Peoples R China;[2]Cent South Univ Forestry & Technol, Food Sci & Technol Coll, Changsha 410004, Hunan, Peoples R China

年份：2024

卷号：450

外文期刊名：FOOD CHEMISTRY

收录：;EI(收录号：20240080126);Scopus(收录号：2-s2.0-85190462220);WOS:【SCI-EXPANDED(收录号:WOS:001333504000001)】；

基金：This research was supported by The Zhejiang province key R & D projects (2021C02014) , and the National Key Research and Development Program of China (2023YFD2201305) .r Development Program of China (2023YFD2201305) .

语种：英文

外文关键词：Camellia saponin; Natural deep eutectic solvent; UPLC-Q-TOF MS; Widely targeted metabolome; KEGG analysis

摘要：Camellia saponins are important by-products of Camellia Oleifer Abel. processing. In this study, an eco-friendly method based on natural deep eutectic solvents (NaDESs, proline and glycerol at a molar ratio of 2:5) was established to extract saponins s from C.oleifera cakes. The content of saponin (702.22 +/- 1.28 mg/g) obtained using NaDES was higher than those extracted using water or methanol. UPLC-Q-TOF MS analysis of chemical structure showed that the difference in the extraction technique alter individual saponins. A widely targeted metabolomic approach and KEGG metabolic pathway analysis showed that the upregulated metabolites in the NaDES-based extract mainly included flavonoids, alkaloids, and phenolic acids; and they were involved in arginine and proline metabolism, metabolic pathways, phenylpropanoid biosynthesis, biosynthesis of secondary metabolites, and flavonoid biosynthesis. The present study proposes a selective substitute for use in the extraction of camellia saponins with composition analysis.