文献类型：期刊文献

中文题名：白蜡虫雄虫真蛹转录组分析

英文题名：Transcriptome Analysis of Male White Wax Scale Pupae

作者：于淑惠[1] 亓倩[1] 孙涛[1] 王雪庆[1] 杨璞[1] 冯颖[1]

第一作者：于淑惠

机构：[1]中国林业科学研究院资源昆虫研究所,国家林业局资源昆虫培育与利用重点实验室,云南昆明650224

年份：2016

卷号：29

期号：3

起止页码：413-417

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：云南省应用基础研究重点项目(2013FA052);林业公益性行业科研专项(201304808,201504302);国家自然科学基金(31572337);国家863计划(2014AA021801)

语种：中文

中文关键词：白蜡虫;蛹;转录组;unigene;热激蛋白

外文关键词：unigene;Ericerus pela;pupae;transcriptome;unigene;heat shock protein

分类号：S899.1

摘要：[目的]蛹期对于白蜡虫雄虫发育和交配具有重要意义,但目前缺乏对于白蜡虫真蛹基因转录情况的分析,本研究旨在获得白蜡虫真蛹转录组数据,了解其转录组特征。[方法]采用Illumina Hi Seq 2000进行高通量测序,并进行生物信息学分析;采用荧光定量PCR(qRT-PCR)检测3个热激蛋白基因(hsp)在不同虫态中的表达动态。[结果]共获得63 957条unigene、63 272个开放阅读框(ORF)、9 561个SSR分子标记,unigene平均长度674 bp,共有14 327条unigene获得了注释,Gene Ontology(GO)注释和Kyoto Encyclopedia of Genes and Genomes(KEGG)注释分析表明,很多基因参与的功能与白蜡虫蛹期的生理特征吻合。[结论]该转录组数据为白蜡虫基因功能鉴定和蛋白组学分析研究奠定了数据基础。
Objective]To obtain the transcriptomic data and characterize the transcriptome of pupal stage of male white wax scale insect,Ericerus pela.[Method]The high-throughput sequencing was performed by Illumina HiSeq 2 000,and bioinformatics analysis were carried out subsequently.Three heat shock protein genes （hsps）were se-lected for expression profile analysis cross different developmental stages using quantitative real-time PCR （qRT-PCR）.[Result]The transcriptomic sequencing generated 63 957 unigenes,63 272 open reading frame （ORF）, and 9 561simple sequence repeat （SSR）markers.The average length of the unigenes was 674 bp.A total of 14 327 unigenes were annotated in different databases.The Gene Ontology （GO）and Kyoto Encyclopedia of Genes and Genomes （KEGG）analysis showed that the putative function of many genes coincided the physiological charac-terization of pupal stage.[Conclusion]The data and analysis of the transcriptome could lay a foundation for further gene functional research and proteomic analysis of E.pela.