文献类型：期刊文献

中文题名：固相萃取/高效液相色谱法测定茶油中的多种天然酚类物质

英文题名：Determination of Phenols in Camellia Oil by High-performance Liquid Chromatography with Solid-phase Extraction

作者：罗凡[1] 费学谦[1] 方学智[1] 王亚萍[1] 王金元[1]

第一作者：罗凡

机构：[1]中国林科院亚热带林业研究所

年份：2011

卷号：30

期号：6

起止页码：696-700

中文期刊名：分析测试学报

外文期刊名：Journal of Instrumental Analysis

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：十一五科技支撑专题项目(2009BADB1B0301);中央级公益性科研院所基本科研业务费专项资金项目(RISF6921)

语种：中文

中文关键词：茶油;酚类物质;高效液相色谱法;固相萃取

外文关键词：camellia oil; phenols ; high-performance liquid chromatography （ HPLC ） ; solid-phase extraction （SPE）

分类号：O657.72;O625.311

摘要：建立了同时分离、检测茶油中23种酚类物质的高效液相色谱方法。比较了液液萃取、固相萃取两种方法的提取效果,并优化了流动相、检测波长、进样量等参数。23种酚类物质在优化条件下均可有效分离,并在0.059~9.115μg/g范围内具有良好的线性关系,相关系数为0.977 6~1.000 0,检出限为0.041~0.379μg/g,相对标准偏差为0.7%~10.1%。23种酚类在茶油中的平均回收率为72%~101%。运用该方法测定了某茶油毛油样品,共检出羟基酪醇、原儿茶酸、儿茶素、4-羟基苯甲酸、绿原酸、香草酸、表儿茶素没食子酸酯、对香豆酸、阿魏9种酚类物质,含量分别为1.178、2.749、1.714、1.287、0.223、0.363、0.219、0.363、0.097μg/g。结果表明该方法准确、可靠,适于不同茶油中酚类物质含量的分析。
A high-performance liquid chromatographic （HPLC） method was established for the separation of various phenols. The extraction efficiency of phenols from camellia oil by the liquid - liquid extraction （LLE） was compared with that by the solid-phase extraction （SPE） method, and the experimental conditions, e.g. mobile phase types, detected wavelength and injection amount of samples were optimized. Under the optimal conditions, all 23 phenols were successfully separated in 60 min. Good linear relationships were obtained in the concentration range of 0. 059 -9. 115 μg/g for 23 phenols, with their correlation coefficients of 0. 977 6 - 1. 000 0. The detection limits were in the range of 0. 041 -0. 379 μg/g. The relative standard deviations were between 0.7% and 10. 1% . The average recoveries of 23 phenols in camellia oil were between 72% and 101%. The proposed method was applied in the determination of phenols in camellia oil sample, and nine phenolics, including hydroxytyrosol, protocatechuic acid, catechins, p-hydroxybenzoic acid, chlorogenic acid, vanillic acid, catechin gallic acid, p-coumaric acid and ferulic acid, were identified with their contents of 1.178, 2.749, 1.714, 1.287, 0.223, 0.363, 0.219, 0.363, 0.097 i^g/g, respectively. The results indicated that the method was accurate and reliable, and was suitable for the analysis of different phenols in various camellia oils.