文献类型：期刊文献

中文题名：芒果采后病原菌的分离及天然抗菌物质的筛选

英文题名：Isolation of Mango Postharvest Pathogenic Fungi and Screening of Natural Plant Antimicrobial Substances Against Them

作者：甘瑾[1] 马李一[1] 张弘[1] 石雷[1] 陈晓鸣[1]

第一作者：甘瑾

机构：[1]中国林业科学研究院资源昆虫研究所

年份：2008

卷号：29

期号：10

起止页码：414-417

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家林业局"948"创新项目(2005-4-C06);"十一五"国家科技支撑计划重点项目(2006BAD06B07)

语种：中文

中文关键词：芒果;采后病害;病原真菌;抑菌作用;天然植物提取液

外文关键词：mango; postharvest pathogen; pathogenic fungi; antibiosis; plant material extracts

分类号：TS201.6

摘要：以云南省元江县的三年芒果为材料,分离并鉴定贮藏过程中引起腐烂的主要病害及病原真菌,同时采用滤纸片扩散法,用25种植物提取液对病原菌进行抑菌实验,以找到对病原菌有抑制作用的天然物质。结果表明,从贮藏中的芒果上分离、鉴定出盘长孢状刺盘孢(Colletotrichum gloeosporioides Penz)、色二孢(Diplodia mangiferae)、大茎点霉(Macrophoma mangiferae Hingoroai & Sharma)、拟茎点霉(Phomposis mangiferae Ahmad)4种病原真菌;确定了对4种病原真菌有抑制作用的天然植物提取液及其最低抑制浓度(MIC),其中对盘长孢状刺盘孢有抑制作用的提取液有100g/L的黄连水提取液、100g/L的高良姜水提取液、6.25g/L的连翘乙醇提取液、50g/L的丁香乙醇提取液和25g/L广霍香乙醇提取液;对色二孢有抑制作用的有100g/L的百部水提取液和12.5g/L的广霍香乙醇提取液;对大茎点霉有抑制作用的是12.5g/L的连翘乙醇提取液、25g/L的广霍香乙醇提取液和50g/L的桉叶乙醇提取液;对拟茎点霉有抑制作用的有25g/L的连翘乙醇提取液、50g/L的丁香乙醇提取液和6.25g/L的广霍香乙醇提取液。
Postharvest pathogenic fungi were iolated and identified from ＂Sannian＂ mango from Yuanjiang county of China＇ s Yunnan province. By the filter paper dispersion method, the antimicrobial effects of 25 kinds of plants extracts by water or ethanol on the pathogenic fungi were investigated respectively. The results showed that the four pathogenic fungi are Colletotrichum gloeosporioides Penz., Diplodia mangiferae, Macrophoma mangiferae Hingoroai ＆ Sharma and Phompsis mangiferae Abroad. The extracts with antimicrobial effects on Colletotrichum gloeosporioides Penz.are 100 g/L water extract of Coptis chinensis Franch, 100 g/L water extract of Alpinia officinarum Hance, 6.25 g/L ethanol extract of Forsytia suspense （Thunb.）Vahl, 50 g/L ethanol extract ofEugenia caryophyllata Thumb. and 25 g/L ethanol extract ofPogostemon cablin Benth; 100 g/L water extract of Stemona jacponica （B1.） Miq and 12.5 g/L ethanol extract of Pogostemon cablin Benth have anfimicrobial effects on Diplodia mangiferae; 12.5 g/L ethanol extract of Forsytia suspense （Thunb.） Vahl, 25 g/L ethanol extract of Pogostemon cablin Benth and 50 g/L ethanol extract of Eucalyptus globulus Labill have antimicrobial effects on Macrophoma mangiferae Hingoroai ＆ Sharma; 25 g/L ethanol extract of Forsytia suspense （Thunb.）Vahl, 50 g/L ethanol extract ofEugenia caryophyllata Thumb. and 6.25 g/L ethanol extract of Pogostemon cablin Benth have antimicrobial effects on Phompsis mangiferae Ahmad.