文献类型：期刊文献

中文题名：高效液相色谱和分光光度法定量分析胭脂红酸的比较

英文题名：Comparison of HPLC and Spectrophotometric Methods for Quantitative Analysis of Carminic Acid

作者：郭元亨[1] 马李一[1] 郑华[1] 张弘[1] 甘瑾[1] 张忠和[1] 张雯雯[1]

第一作者：郭元亨

机构：[1]中国林业科学研究院资源昆虫研究所,国家林业局资源昆虫培育与利用重点实验室

年份：2009

期号：18

起止页码：303-306

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金："十一五"国家科技支撑计划项目(2006BAD06B07);科技部成果转化项目(2007GB24320425)

语种：中文

中文关键词：高效液相色谱;分光光度法;胭脂红酸;定量分析

外文关键词：HPLC; spectrophotometry; carminic acid; quantitative analysis;

分类号：TS202.3;S899.3

摘要：利用高效液相色谱和分光光度法定量分析胭脂虫红色素样品中的胭脂红酸,以Sigma公司生产的胭脂红酸为标样,分别用高效液相色谱测峰面积和用分光光度法测吸光度,分别绘制标准曲线方程得:Y=7.8260X,R2=0.9988和Y=0.0137X,R2=0.9997浓度在9.5～95.0μg/ml的范围内线性相关性良好。并分别用高效液相色谱法和分光光度法对10种色素样品进行定量分析,分光光度法所得结果较高效液相色谱结果偏高,但两种方法所得结果经单因素方差分析,显著性指标P值为0.904,即两种方法测定结果差异性不显著。两种方法都可以用于定量分析胭脂虫红色素样品中的胭脂红酸。
Using carminic acid produced by Sigma company as a reference substance, HPLC and spectrophotometric methods were applied to quantify carminic acid in cochineal. Two linear equations were made respectively for peak area measured by HPLC and absorbance at 494 nm measured by spectrophotometry as follows： Y=7.8260X, R^2=0.9988 and Y=0.0137X, R^2=0.9997, in the concentration range of 9.5—95.0 μg/ml. Using the two methods for quantitative analysis of 10 commercial or self-prepared cochineal samples, all results measured by spectrophotometry were slightly higher than those by HPLC, but there was no statistical difference between them. In conclusion, both the two methods can be used for quantitative analysis of carminic acid in cochineal samples.