文献类型：期刊文献

中文题名：银离子络合法分离纯化余甘子核仁油中α-亚麻酸

英文题名：Separation and Purification of α-Linolenic Acid from Phyllanthus emblica L. Seed Oil by Silver Iron Complexation

作者：葛双双[1] 张雯雯[1] 李坤[1] 冯颖[1] 甘瑾[1] 郑华[1] 张弘[1]

第一作者：葛双双

通信作者：Zhang, Hong|[a00050201897da7fe7284]张弘;

机构：[1]中国林业科学研究院资源昆虫研究所国家林业局特色森林资源工程技术研究中心

年份：2017

卷号：38

期号：14

起止页码：213-220

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD;;EI(收录号：20173704158089);Scopus;北大核心:【北大核心2014】；CSCD:【CSCD_E2017_2018】；

基金："十三五"国家重点研发计划重点专项(2016YFD0600806);中央级公益性科研院所基本科研业务费专项(riricaf2015003M)

语种：中文

中文关键词：余甘子核仁油;α-亚麻酸;Ag+络合

外文关键词：Phyllanthus emblica L. seed oil; α-linolenic acid; silver iron complexation

分类号：TQ645.6;TS225.1

摘要：利用Ag^+络合技术分离、纯化余甘子核仁油中α-亚麻酸,旨在获得高纯度的α-亚麻酸。通过单因素试验考察了AgNO_3溶液浓度、甲醇溶液体积分数、络合时间、络合温度对α-亚麻酸纯度及回收率的影响。在单因素试验基础上,以α-亚麻酸纯度及回收率为响应值,进行Box-Behnken试验设计,优化得到的最佳工艺条件为络合温度为0℃、AgNO_3溶液浓度为2.29 mol/L、甲醇溶液体积分数为38.00%、络合时间为1.93 h,在此工艺条件下,α-亚麻酸纯度及回收率分别为93.30%及73.37%。对络合后的AgNO_3进行回收并二次络合,结果表明:AgNO_3的回收率达93.83%,回收的Ag^+具有较好的络合效果,在整个络合工艺中并未引入Ag^+,因此,该方法不仅实现了对余甘子核仁油中α-亚麻酸的分离、纯化,而且降低了络合工艺的操作成本,同时具有一定安全性,为余甘子核仁油的工业化应用提供理论依据。
The objective of this research was to purifyα-linolenic acid from Phyllanthus emblica L.seed oil by usingsilver iron complexation in order to obtain high purity and yield ofα-linolenic acid.Firstly,the effects of silver nitrateconcentration,volume fraction of methanol,complexation time and complexation temperature on the purity and yield ofα-linolenic acid were investigated by one-factor-at-a-time method.Afterwards,three independent variables were selected outof the four process parameters for the optimization experiments which were done using Box-Behnken design with the purityand yield ofα-linolenic acid as response variables.The results indicated that the optimal process parameters were obtainedas follows:complexation temperature,0℃;silver nitrate concentration,2.29mol/L;methanol volume fraction,38.00%;and complexation time,1.93h,under which the purity and yield ofα-linolenic acid were93.30%and73.37%,respectively.The remaining silver nitrate was recovered for a second complexation.The recovery of silver nitrate was93.83%and therecovered Ag+still had good complexation effect.Particularly,exogenous Ag+was not introduced in the whole complexationprocess.Therefore,this method allowed successful separation and purification ofα-linolenic acid from P.emblica L.seedoil while reducing the operating cost.Meanwhile,the method of silver iron complexation was safe and helpful for industrialapplication of P.emblica L.seed oil.